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Sample collection and processing Venous blood samples (10 mL) were obtained from the antecubital vein of each participant

OECD (2019) advised utilising the juvenile stage when testing for acute toxicity

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The liquid volume controls concentration: more liquid creates a less concentrated solution and a larger volume reading for the same target amount

Degraded peptide may have lost its copper-binding capacity, creating a solution of free copper rather than beneficial GHK-CU complex

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