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- Discard supernatant and resuspend at 1 x 10E8 cells per milliliter in RPMI 1640 / 1% FCS How do I carry out tissue digests to obtain a single-cell suspension before using Dynabeads magnetic beads for cell isolation

Yes, glutathione can typically be taken daily

BMC Immunol (2015) 16:35

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IRAK-M regulates the inhibition of TLR-mediated macrophage immune response during late in vitro Leishmania donovani infection

A pragmatic alternative is to prepare smaller vials from your reconstituted stock and freeze them as single-use aliquots at 20C, minimizing repeated freeze-thaw exposure to your main vial