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Cells were washed in FACS buffer (2% FBS in PBS), fixed and permeabilized using the Cytofix/Cytoperm kit, and stained intracellularly using antibodies for 30 min at 4 C in the dark

The nucleus accumbens: mechanisms of addiction across drug classes reflect the importance of glutamate homeostasis

When you breathe slowly and deeply, these sensors trigger a relaxation response
