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For example, the Carotene and Retinol Efficacy Trial (CARET), a study of beta-carotene and retinyl palmitate supplementation, was stopped prematurely due to an increase in lung cancer risk and death from lung cancer in participants who received the intervention [109]

This obviously raises a question on the applicability of low-resolution techniques for the analysis of these phenomena

MCF-7 cell lines (ATCC, USA) were cultured in RPMI 1640 media (EuroClone S.p.A., Italy) supplemented with 1% penicillin-streptomycin, 1% L-Glutamine, and 10% fetal bovine serum (FBS) and incubated in a 37 C incubator under 5% CO2 atmosphere

The velocity was calculated by multiplying the fitted rate constant by the fitted reaction amplitude

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Inouye et al., 1985